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Developmental changes in the constitutive and inducible expression of cytochrome p450 3a2

Identifieur interne : 001808 ( Main/Exploration ); précédent : 001807; suivant : 001809

Developmental changes in the constitutive and inducible expression of cytochrome p450 3a2

Auteurs : Matthew C. Wright ; Robert J. Edwards ; Michel Pimenta [Portugal] ; Vera Ribeiro [Portugal] ; Gurpreet S. Ratra ; Maria Celeste Lechner [Portugal] ; Alan J. Paine

Source :

Biochemical Pharmacology [ 0006-2952 ] ; 1997.

RBID : ISTEX:3DA8851FEEFDF5284B909CF7241269257FD2D65F

English descriptors

Entity :
- org : Bartholomew’s Hospital Medical College, Charterhouse Square, London, Department of Toxicology, St, Elsevier Science Inc, Junta National Investiga, PORTUGAL ABSTRACT, Sigma Chemical Co., UK WI.
- pers : Alan J. Paine, Charles River, Henry Strohel, Maria Celeste, Matthew C. Wright, Michel Pimenta, Robert J. Edwards, S. Ratra, Vera Ribeiro.
- place : Bournemouth, Poole, Portugal, UK.
KwdEn :
- CYP3A2, cytochrome P450, gene expression, metyrapone, rat liver, steroid 6β-hydroxylase.
Teeft :
- Adult male rat, Adult rat, Arch biochem biophys, Biochem, Biol chem, Biophys, Cdna, Control rat, Cyp3a, Cyp3a protein, Cyp3al, Cytochrome, Developmental regulation, Elsevier science, Female liver, Female rat, Gene expression, Glucocorticoid, Greater electrophoretic mobility, Hepatic, Hepatic cytochrome, Liver microsome, Male rat, Metyrapone, Microsomal, Microsomal protein, Mrna, Mrna expression, Other cyp3a protein, Previous report, Rat, Separate animal, Terminus, Western blot.

Abstract

Abstract: Using a CYP3A2-specific oligonucleotide and an antipeptide antibody raised against the C terminus of CYP3A2 (VINGA) it is demonstrated that metyrapone administration to adult (12 weeks old) but not immature (3 weeks old) male Sprague Dawley rats induces the hepatic expression of CYP3A2 mRNA and protein. The consdtutively expressed level of CYP3A2 protein in adult male rats is markedly lower than the levels expressed in immature rats as determined using the anti-VINGA antibody, in contrast to previous reports using antibodies that do not discriminate between CYP3A forms. Hepatic microsomal CYP3A2 protein expression, examined between 3 and 15 weeks of age, is extinguished between 9 and 12 weeks of age in contrast to immunoreactive CYP3A protein (determined using a nonselective antibody) and CYP3A-dependent androstenedione 6β-hydroxylase activity. These data suggest that the regulation of the induction of CYP3A2 is developmentally controlled and that the major expressed adult form(s) of constitutively expressed CYP3A is not CYP3A2.

Url:

https://api.istex.fr/ark:/67375/6H6-VDQRMG76-K/fulltext.pdf

DOI: 10.1016/S0006-2952(97)00264-5

Affiliations:

Portugal

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: Using a CYP3A2-specific oligonucleotide and an antipeptide antibody raised against the C terminus of CYP3A2 (VINGA) it is demonstrated that metyrapone administration to adult (12 weeks old) but not immature (3 weeks old) male Sprague Dawley rats induces the hepatic expression of CYP3A2 mRNA and protein. The consdtutively expressed level of CYP3A2 protein in adult male rats is markedly lower than the levels expressed in immature rats as determined using the anti-VINGA antibody, in contrast to previous reports using antibodies that do not discriminate between CYP3A forms. Hepatic microsomal CYP3A2 protein expression, examined between 3 and 15 weeks of age, is extinguished between 9 and 12 weeks of age in contrast to immunoreactive CYP3A protein (determined using a nonselective antibody) and CYP3A-dependent androstenedione 6β-hydroxylase activity. These data suggest that the regulation of the induction of CYP3A2 is developmentally controlled and that the major expressed adult form(s) of constitutively expressed CYP3A is not CYP3A2.</div>
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Developmental changes in the constitutive and inducible expression of cytochrome p450 3a2

Developmental changes in the constitutive and inducible expression of cytochrome p450 3a2

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri